Comparison of vikia rotaadeno to ideia with 189 stool samples. Immunochromatographybased diagnosis of rotavirus infection. Pdf sensitivity and specificity of the vikia r rota. Update on schemes and the future uk neqas microbiology. Fecal rotaadeno duo antigen rapid test kit abin5186. Tests for the rapid diagnostic of rotavirus english. Diagnostic accuracy of seven commercial assays for rapid detection of group a rotavirus antigens jerome kaplon, aceline fremy, sylvie pillet,b lucile mendes martins,c katia ambertbalay,a serge l. Children aged 5 years and under 59% samples had a virus 36. Vikia rotaadeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotavirus and adenovirus. L, zaragoza, spain and vikia rota adeno biomerieux sa, lyon, france were retrospectively evaluated using realtime pcr as reference test. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaoundecameroon ngum vn. Interestingly, the specificity of the vikia rotaadeno assay was 100%.
The full text of this article is available in pdf format. Vikia rotaadeno biomerieux, france streptococcus quickvue inline strep a test quidel, usa, and urine dipsticks urilab h10. Detection of rotavirus, norovirus and adenovirus by molecular. The incidence of rotavirus and adenovirus infections among.
Rapid diagnosis of the infectious agent avoids unnecessary antibiotic treatment and leads to rapid isolation of infected patients. Herve crehalet, francois vandenesch, anne marie freydiere. Pdf objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and. Tests for the rapid diagnostic of rotavirus english translation. The pathflow rota adeno combi test cassette is a rapid chromatographic immunoassay for the qualitative detection of rotavirus and adenovirus in human faeces, providing results from 10 minutes offering an easytouse, rapid alternative. Genotype distribution and phylogenetic analysis of. Clostridium difficile infection in polish pediatric. Acute diarrhea in tropical countries can be caused by a broad spectrum of viral, parasitic and bacterial enteropathogens. Rotavirus a and adenovirus in samples of human faeces were compared. Enteric adenovirus kit pcr positive negative sensitivity % specificity % ppv % npv % vikia positive 8 0 negative 2 190 80 100 100 98. Evaluation of the bionexia rotaadeno rapid test h briand 1, l ji 2, j morgand 1, l thery 3, m bidalot 3, k ambertbalay 3, j kaplon 3 a duraffourgcollomb 1, j. The study protocol was approved by the institutional ethical. After informed consent and epidemiologic and clinical data had been obtained, a fecal specimen was collected from each child.
Enteric prevalence of rotavirus and adenovirus in under five. Performance analysis of two immunochromatographic assays. The main objective of this study was to identify rotavirus and adenovirus in under five children predisposed with acute diarrhea in yaoundecameroon using vikia rotaadeno rapid test kit biomerieux, france. Research tropheryma whipplei in children with gastroenteritis. Letters rotavirus epidemiology in bangui, central african and vp7 and vp4 were amplified by republic, 20081 to the editor. In the study, one hundred children less than 5 years of age with acute gastroenteritis admitted to sulaimanipaediatric hospital were studied. A total of 250 infants and young children with diarrhea 159 male and 91 female, mean age 8. Biomerieux vikia rotaadeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom. If no stool sample was obtained during the baseline visit, parents were requested to collect a stool sample and bring it into the health care center within four days. Vikia rota adeno kit is a qualitative test based on the association of monoclonal antibodies. This is a qualitative test based on the immunochromatography technique. Performance analysis of two immunochromatographic assays for the.
Adiogo d, fokunang cn, gonsu kh, tembefokunang ea, abena omt. Enteric prevalence of rotavirus and adenovirus in under. Multicenter prospective study on the burden of rotavirus. Interestingly, the specificity of the vikia rota adeno assay was 100%. Vikia rota adeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotaviruses and adenoviruses, which are responsible for the majority of pediatric diarrhea cases. Vikia rota and adeno biomerieux france is a qualitative test based on the immunochromatography technique ict. The elisa test, ideia from dako, considered as the reference method was blindly performed by biomerieux laboratory. In our study, the evaluation was done after optimization of the manufacturing process for vikia rotaadeno biomerieux personal communication, which may explain the difference between the australian study and this study. This term was created for, but is not limited in use to, the biomerieux vikia rota adeno one step combo card test, which is intended for the dual detection of rotaviruses and adenoviruses in a single stool extract.
The multiplex assays were clinically evaluated using 239 fecal samples submitted to our laboratory over a 1year period for the routine detection of rotavirus andor adenovirus antigens using the vikia rotaadeno test biomerieux, boxtel, the netherlands. Tutkimuksen tarkoituksena oli vertailla ka ytossa olevaa diarlex mb vieritestia rida quick ja vikia vieritesteihin. Group a rotaviruses rvas are the primary cause of acute gastroenteritis. Rotavirus epidemiology ods in bangui, central african. Pdf diagnostic accuracy of vikia rotaadeno and premier. In the analysis of rotavirus, the molecular and immunological methods had an agreement of 83%, while for adenovirus the agreement. Rotavirus infection in children and adult patients attending. Pred uporabo dobro preberite tudi originalna navodila. Patientswith ibd were treated with sulfasalazine or mesalazine, azathioprine, cyclosporine, steroids and infliximab according to accepted clinical protocols. Specimens were obtained from 1,350 hospitalized patients and. Rapid communications evidence of falsepositive results in a.
Clostridium difficile infection in polish pediatric outpatients with inflammatory bowel disease d. We conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Serodetection of rotavirus and adenovirus gastroenteritis co. Rotavirus was detected every month during the period of the study, with peak of positivity between june and august 35%. Edelleen ripulitauteihin kuolee koko maai lmassa vuosittain 2,5 miljoona lasta. Rotavirus epidemiology in bangui, central african republic. Oct 10, 2016 one stool sample was collected for rotavirus testing vikia rotaadeno immunochromatographic test biomerieux from each child sensitivity 96. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaounde. Biomerieux vikia rota adeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom.
A total of 3,768 stool samples were received during the study period, 55. After receiving concerns regarding this test, company subsequently performed a parallel evaluation of the diagnostic accuracy of vikia rota adeno. Biomerieux vikia rotaadeno vzporedni test za rotaviruse in. Diagnostic accuracy of vikia rotaadeno and premier rotaclone tests for the. Trends of viral gastroenteritis in the australian capital. Diagnostic accuracy of vikia rotaadeno and premier. For adenovirus, vikia rotaadeno kit produced 1 positive result 5. Of the 24 hrv positive samples, 19 were confirmed with the multiplex pcr assay and 4 additional positive samples were detected, making a total of 9 discrepant samples. The samples were analyzed using vikia rotaadeno test kit biometrieux. Results obtained by the vikia rotaadeno test revealed that 100 40% of these children were infected with rotavirusa, mostly male children 61100, p vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Adenovirus diagnostic testing market growth analysis 2020 by. The aim of this study was to determine the incidence and clinical significance of rotavirus and enteric adenovirus gastroenteritis and also to determine possible risk factors for rotavirus and adenovirus gastroenteritis using new simple rapid screening test vikia rota and adeno.
Vikia biomerieux, kcombi set bioconnections, rida quick rbiopharm and quickstrip savyon diagnostics. We therefore reexamined samples initially testing pos itive in the vikia rotaadeno ict with other commercially available elisa rotavirus assays and, for a subset of specimens, by rtpcr. Vikia rotaadeno and 10 56 % were positive when analysed by rtpcr table 1. L, zaragoza, spain and vikia rotaadeno biomerieux sa, lyon, france were retrospectively evaluated using realtime pcr as reference test. We excluded rotavirus specimen tests where age or sex was missing n21. Adeno ja rotaviruksia osoittavien vier i testien vertailu. Acute diarrheal illness was defined as occurrence of. All reagents and stool samples were allowed to reach room temperature before use. Diagnostic accuracy of seven commercial assays for rapid.
This observation underlines the importance of regular assess. Rapid test for the qualitative dual detection of rotavirus and adenovirus in human stools. Pdf sensitivity and specificity of the vikia r rotaadeno. Rotavirus infection in children and adult patients. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse. M dugua 1 introduction results acute gastroenteritis age is one of the most common diseases in humans and. If the sample is adeno positive, we recommend to further confirm the serotype result with 4041 adeno strip. From january 2006 to july 2009, 467 samples of faeces from individuals with gastroenteritis. Loinc 803767 rotavirus and adenovirus ag panel stool.
Biomerieux vikia rotaadeno vzporedni test za rotaviruse. Rotavirus epidemiology ods in bangui, central african and. We conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Detection and molecular characterisation of adenovirus in. M dugua 1 introduction results acute gastroenteritis age is one. Feb 17, 2020 the pdf for the study can be requested using the following link. Vikia rota adeno biomerieux diarlex mb fumouze b the two immunochomatographic tests were performed in the clinical laboratory of the debrousse hospital.
Orion diarlex rota microgen rotascreen eia in house pcr biomerieux vikia coris combistrip biokit biorapid novamed rotastick rbiopharm ridaquick rota certest rota adeno card certest rota card atlas medical rota adeno biosigma fast step oxiod prospect quadratech check4 rotavirus rbiopharm ridaquick kombi. Ict assay biomerieux, marcy letoile, france, while excellent specificity was. Rapid communications evidence of falsepositive results in. Oct 23, 2017 europe pmc is an archive of life sciences journal literature. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse. Sensibilite et specificite du test immunochromatographique vikia. Rotavirus infection in children and adult patients attending in a. Infection with group a rotavirus is among the lead. Diagnosis of viral gastroenteritis by simultaneous detection. The study was part of a rotavirus surveillance project in niger. Vikia rotaadeno is a simple rapid test used as an aid in the immediate diagnosis of viral gastroenteritis by rotaviruses and adenoviruses. Performance analysis of two immunochromatographic assays for. Europe pmc is an archive of life sciences journal literature.
The vikia rotaadeno test gave a positive result in 24239 10. This test uses immunological reactions performed on a test strip by migration. Accurate detection of rotavirus is essential for prevention and control of rotavirus outbreaks and disease monitoring. Many diagnostic laboratories in australia use the vikia rotaadeno assay for detection of rotavirus in faecal specimens. Rapid diagnosis of rota adenoviruses for acute gastroenteritis in hospitalized children under 4 years old, baghdad razaq hadi eissa alsayidi1. M dugua 1 introduction results acute gastroenteritis age is one of the most common diseases in humans and continues to be a cause of high morbidity and mortality in children.
Navodila za uporabo instrumenta naj bodo zmeraj v blizini naprave. Oct 23, 2017 we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Adenovirus diagnostic testing market growth analysis 2020. Sensitivity and specificity of the vikia r rotaadeno immunochromatographic test biomerieux and the elisa ideia tm rotavirus kit dako compared to genotyping. In terms of frequency, rotavirus was detected in10 10% of the samples followed by adenovirus type 4041 44%.
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